Antibiotic resistance poses a serious public health threat that can lead to decreased effectiveness of many therapies, increased morbidity, prolonged hospital stays, higher mortality rates, and additional costs for healthcare systems. Irrational use of antibiotics can result from a lack of proper knowledge about antibiotic therapy and the risks associated with antibiotic resistance, both among medical staff and patients. The main objective of the study was to assess the opinions of medical personnel on the risks associated with antibiotic resistance. Medical staff consider antibiotic resistance to be a fairly serious issue, although not all agree on its severity. The risk of antibiotic resistance is significantly more seriously assessed by doctors and nurses, as well as by those with specializations or undergoing specialized training. Knowledge about antibiotic resistance should be further disseminated among all groups of medical personnel.